DS/CEN/TS 16835-3:2015 ( Tilbagetrukket )

Molekylærbiologiske in vitro-diagnostiske undersøgelser - Specifikationer for præanalytiske processer for venøst fuldblod - Del 3: Oprenset cirkulerende cellefrit DNA fra plasma


Status:
Tilbagetrukket
Type:
Standard
Sprog:
Engelsk
Kategori:
Pris fra:
kr. 341,00

Beskrivelse

This Technical Specification recommends the handling, documentation and processing of venous whole blood specimens intended for circulating cell free DNA (ccfDNA) analysis during the preanalytical phase before a molecular assay is performed. This Technical Specification covers specimens collected by venous whole blood collection tubes. This Technical Specification is applicable to molecular in vitro diagnostic examinations (e.g. in vitro diagnostic laboratories, laboratory customers, in vitro diagnostics developers and manufacturers, institutions and commercial organizations performing biomedical research, biobanks, and regulatory authorities).

Blood ccfDNA profiles can change significantly after blood collection from the donor (e.g. release of genomic DNA from white blood cells, ccfDNA fragmentation and ccfDNA quantity change). Special measures need to be taken to secure good quality blood samples for ccfDNA analysis and storage.

Different dedicated measures need to be taken for preserving blood genomic DNA. These are not described in this Technical Specification. Blood genomic DNA is covered in FprCEN/TS 16835-2, Molecular in vitro diagnostic examinations – Specifications for pre-examination processes for venous whole blood – Part 2: Isolated genomic DNA

NOTE – CcfDNA obtained from blood by the procedures suggested in this document can contain DNA present in exosomes [3] [4].

Pathogen DNA present in blood is not covered by this Technical Specification.


Antal sider: 17

Udgivet: 2015-12-10

Godkendelsesdato: 2015-11-05

Tilbagetrækningsdato: 2019-10-28

Internationale relationer : CEN/TS 16835-3:2015 IDT

ICS: 11.100.30 - Analyse af blod og urin

Varenummer: M296075


Udvalg og komiteer

Komite

  • CEN/TC 140
  • CEN/TC 140/WG 3

Dansk udvalg

DS/S-260