DS/EN 15607:2009

Foodstuffs - Determination of d-biotin by HPLC


Status:
Current
Type:
Standard
Language:
English
Subject:
Price from:
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Scope

This European Standard specifies a method for the determination of the mass fraction of d-biotin by high performance liquid chromatography (HPLC). The method has been validated in an inter-laboratory test on fortified and non-fortifed samples such as cereal breakfast powder, infant milk powder, lyophilized green peas with ham, lyophilized chicken soup and on nutritive orange juice, at levels from 16 μg/100 g to 200 μg/100 g. For further information on the validation data, see Annex B. NOTE 1 d-biocytin can also be estimated by this method. But none of the samples used for the validation step contained d-biocytin. Nonetheless the recovery rate is more than 90 % for d-biotin and d-biocytin, see [2] and [3]. NOTE 2 The method underestimates the real biotin content when used for samples containing egg.

Number of pages: 20

Published: 2009-07-24

Date of approval: 2009-06-23

International relationships : EN 15607:2009 IDT

ICS: 67.050 - General methods of tests and analysis for food products

Item number: M216193


Committees

Committee

  • CEN/TC 275/WG 9

Danish committee

DS/S-291