1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in traditional top-loading automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.Note 1: This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading or top-loading, low water high efficiency automatic clothes washing operations.1.2 This test method is intended to compliment Test Method E2406 and is to be used in the cases where this test method is determined to be the worse case scenario for product usage.1.3 Knowledge of microbiological techniques is required for these procedures.1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.)1.5