1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in front loading high efficiency (HE) automatic clothes washing operations that typically utilize very low wash water volumes. This test method is designed to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.1.2 This test method is intended to compliment Test Method E2274 and is to be used in the cases where this test method is determined to be the worse case scenario for product usage.Note 1: Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry detergent/additives for use in traditional high wash water volume automatic clothes washing operations.1.3 Knowledge of microbiological techniques is required for these procedures.1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropr